NDLI: Molecular and genetic analyses of geographic variation in isolates of Phoma macrostoma used for biological weed control.

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Molecular and genetic analyses of geographic variation in isolates of Phoma macrostoma used for biological weed control.

Content Provider	Semantic Scholar
Author	Zhou, Lecong Chen, Charles Keri, Mario
Copyright Year	2005
Abstract	Molecular and genetic approaches were used to evaluate the genetic relatedness among isolates of the fungus Phoma macrostoma Montagne originating from Canada and Europe and to other species in the genus Phoma. Distinct differences were observed in genetic variation among nine species of the genus Phoma. Randomly amplified polymorphic DNA (RAPD) revealed the presence of intraspecific genetic variation among the isolates of P. macrostoma, with the isolates being used for biological weed control being distributed in a distinct phylogenetic cluster. Additional variation within the biocontrol isolate cluster in P. macrostoma was revealed by pulsed field gel electrophoresis (PFGE), which showed that biocontrol isolates generated two different chromosomal profiles, however the profiles did not relate to their Canadian ecozone origin. Mating studies showed that biocontrol isolates of P. macrostoma from Canada did not produce sexual reproductive structures and were incapable of crossing. These studies also confirmed that no obvious differentiation exists among the biocontrol isolates of P. macrostoma from Canadian Ecozones 3 and 4.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://dzumenvis.nic.in/Pathogens/pdf/Molecular%20and%20genetic%20analyses%20of%20geographic.pdf
PubMed reference number	16392250v1
Volume Number	97
Issue Number	3
Journal	Mycologia
Language	English
Access Restriction	Open
Subject Keyword	Didymella macrostoma Electrophoresis, Gel, Pulsed-Field Ethanol 0.62 ML/ML Topical Gel Fungi Gel Electrophoresis (lab technique) Genus Phoma Random Amplified Polymorphic DNA Technique Reproduction Variation (Genetics)
Content Type	Text
Resource Type	Article

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