NDLI: Molecular epidemiological analysis of Mycoplasma bovis isolates from the United Kingdom shows two genetically distinct clusters.

Please wait, while we are loading the content...

Molecular epidemiological analysis of Mycoplasma bovis isolates from the United Kingdom shows two genetically distinct clusters.

Content Provider	Semantic Scholar
Author	Mcauliffe, Laura Kokotovic, Branko Ayling, Roger David Nicholas, Robin Ashley John
Copyright Year	2004
Abstract	Mycoplasma bovis is an important veterinary pathogen causing pneumonia, arthritis, and mastitis in infected cattle. We investigated the genetic diversity of 53 isolates collected in the United Kingdom between 1996 and 2002 with pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), and random amplified polymorphic DNA (RAPD) analysis. In addition, the influence of variable surface protein (Vsp) profiles on the profiles generated with molecular typing techniques was studied. Both AFLP and RAPD separated the isolates into two distinct groups, but PFGE showed less congruence with the other techniques. There was no clear relationship between the geographic origin or year of isolation of the isolates and the profiles produced. No correlation between Vsp profiles and any of the molecular typing techniques was observed. We propose that RAPD and AFLP provide valuable tools for molecular typing of M. bovis.
Starting Page	1
Ending Page	27
Page Count	27
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jcm.asm.org/content/42/10/4556.full.pdf
PubMed reference number	15472309v1
Alternate Webpage(s)	https://doi.org/10.1128/JCM.42.10.4556-4565.2004
DOI	10.1128/JCM.42.10.4556-4565.2004
Journal	Journal of clinical microbiology
Volume Number	42
Issue Number	10
Language	English
Access Restriction	Open
Subject Keyword	ELECTROSURGICAL DEVICE Electrophoresis, Gel, Pulsed-Field Epidemiology Ethanol 0.62 ML/ML Topical Gel Gel Electrophoresis (lab technique) Membrane Proteins Molecular Typing Mycoplasma bovis Pathogenic organism Pneumonia Random Amplified Polymorphic DNA Technique Variation (Genetics) efmoroctocog alfa 1 UNT Injection [Eloctate]
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in