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Detection of micrometastases in lymph nodes using reverse transcription polymerase chain reaction (RT-PCR) for cytokeratin 20 (CK-20)--a pilot study.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wijesuriya, Suraj Ruwan Kuruppuarachchi, K. G. R. Weerasinghe, Anura Hewavisenthi, Janaki Deen, Kemal Ismail |
| Copyright Year | 2010 |
| Abstract | OBJECTIVES The aim of the study was to detect micrometastases in lymph nodes in patients with rectal cancer following neoadjuvant therapy, staged node negative by routine histology. PATIENTS AND SETTING Mesenteric lymph nodes from patients who have undergone neoadjuvant therapy for rectal cancer were harvested during surgery. Nodes were bisected and one half was sent for haematoxylin and eosin (H&E) staining and evaluated by a single pathologist. The other half was examined for CK20 by RT-PCR. The technique was validated by testing mesenteric lymph nodes with known metastases and nodes from patients without cancer. Twenty one lymph nodes from 6 patients (median age 46 years, range 25-55) which were negative for tumour deposits by H & E stain were assessed for micro-metastases. RESULTS All 21 nodes which were histologically negative for metastases were positive for micrometastases. Two nodes with known metastases were positive for CK20 and 3 nodes from non cancer patients were negative for CK20. CONCLUSIONS Detection of CK20 is accurate in identification of rectal cancer micro-metastasing to lymph nodes. Assessment of nodes by H & E histology risks under staging. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://cmj.sljol.info/article/download/2290/1886/ |
| PubMed reference number | 21033302v1 |
| Volume Number | 55 |
| Issue Number | 3 |
| Journal | The Ceylon medical journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cytokeratin Eosine Yellowish Hematoxylin KRT20 gene KRT20 wt Allele Lymph node of mesentery Lymphadenopathy Neoadjuvant Therapy Neoplasms Patients Port-Wine Stain Real-Time Polymerase Chain Reaction Rectal Carcinoma Rectal Dosage Form Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Staining method TUBE,RECTAL,24FR,PLASTIC B#6510 lymph nodes |
| Content Type | Text |
| Resource Type | Article |
