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The use of molecular profiling of early colorectal cancer to predict micrometastases.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Saha, Sukamal Kuo, Christine Ye, Xing |
| Copyright Year | 2002 |
| Abstract | BACKGROUND Approximately one third of node-negative colorectal cancers (CRCs) recur, suggesting the failure to detect occult disease. Lymphatic mapping followed by focused analysis of the sentinel node is highly accurate in identifying micrometastases. HYPOTHESIS Because aberrant genetic changes occur early in tumor progression and are associated with lymphatic metastases, we hypothesized that the molecular profiling of specific tumor markers in the primary tumor might predict that tumor's metastatic potential. DESIGN A prospective patient series. PATIENTS AND INTERVENTIONS Forty consecutive patients with early CRC underwent lymphatic mapping after subserosal injection of 1 mL of isosulfan blue dye. All lymph nodes were examined by hematoxylin-eosin (HE) staining, and multiple sections of each sentinel node were examined by HE and cytokeratin immunohistochemistry (CK-IHC) staining. Primary tumors were analyzed for p53 expression using IHC staining and for beta-human chorionic gonadotropin (beta-hCG), hepatocyte growth factor receptor (c-Met), and universal melanoma antigen (uMAGE) messenger RNA expression using reverse-transcriptase polymerase chain reaction and electrochemiluminescence. RESULTS Nine patients (23%) had positive nodes by routine HE staining. Of the remaining 31 patients with negative nodes on HE staining, 8 tumors (26%) were upstaged by CK-IHC identification of occult micrometastases. There was a direct correlation between the number of markers and the T stage (P =.001). The expression of p53, beta-hCG, c-Met, and uMAGE in primary tumors was significantly higher in the presence of nodal micrometastases vs no metastases (P =.03). CONCLUSIONS Sentinel lymphatic mapping is an accurate method of detecting micrometastases in CRC. Molecular profiling of primary CRC tumors, similar to that used for breast cancer, may be important in predicting metastatic potential and determining which patients may benefit from adjuvant therapy. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://archsurg.jamanetwork.com/pdfaccess.ashx?url=/data/journals/surg/5375/sws1001.pdf |
| Alternate Webpage(s) | https://archsurg.jamanetwork.com/journals/SURG/articlepdf/213243/sws1001.pdf |
| PubMed reference number | 12470104v1 |
| Volume Number | 137 |
| Issue Number | 12 |
| Journal | Archives of surgery |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anatomic Node Chorionic Gonadotropin, beta Subunit, Human Colorectal Cancer Colorectal Carcinoma Cytokeratin Dyes Electrochemiluminescence Eosine Yellowish Gonadotropins Growth Factor Receptors Hematoxylin and eosin stain method Immunohistochemistry Lymphatic Diseases Lymphatic Metastasis Mammary Neoplasms Molecular Diagnostic Techniques Molecular Profiling Patients Pharmaceutical Adjuvants Polymerase Chain Reaction RNA Receptors, LH Sentinel node (disorder) Sinoatrial Node Stage I Carcinoma of Bladder Staining method Transcription, Genetic Tumor Markers Tumor Progression iso-sulfan blue lymph nodes primary tumor |
| Content Type | Text |
| Resource Type | Article |
