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01112 12 3 4 5 6 7 8 9101112 1 2 3 4 5 6 7 8 SttJiyM A-G-string (-antibody ) B ' G " string ( + antibody ) C : Spl
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lewis, Catherine D. Clark, S. P. R. Felsenfeld, Gary Gould, Hannah |
| Copyright Year | 2007 |
| Abstract | The promoter region of the chicken aduh p-globin gene contains a sequence of 16 deoxyguanosine residues located at a nucleosome boundary in tissues where the gene is inactive. In definitive erythrocytes that express the p-globin gene, the nucleosome is displaced, the G-string and adjacent sequences are occupied by sequencespecific DNA-binding proteins, and a nuclease hypersensitive domain is generated in this region. To gain insight into the role of the G-string in this series of events, we have examined the proteins that bind to it. Using the gel mobility shift assay and a monoclonal antibody that blocks specific binding to the G-string, we have identified a specific protein, BGPl, that is found only in chicken erythroid cells and appears at the same time, or shortly before, the changes in chromatin structure. The antibody interacts strongly with BGPl and cross-reacts weakly with Spl. Although both BGPl and Spl require Zn̂ + for their DNA-binding activity, these proteins differ in their binding-site specificities, chromatographic properties, and molecular weights. In contrast to Spl, which is found in a wide variety of cell types, BGPl is restricted to erythrocytes and is most abundant in definitive erythrocytes. Thus, its presence corresponds to the tissueand stage-specific occupancy of the G-string in vivo. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://genesdev.cshlp.org/content/2/7/863.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
