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MiR-199a/b-5p inhibits hepatocellular carcinoma progression by post-transcriptionally suppressing ROCK1
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhan, Yangyang Zheng, Nanxin Teng, Fei Bao, Leilei Liu, Fang Zhang, Mingjian Guo, Meng Guo, Wenyuan Ding, Guo-Shan Wang, Quanxing |
| Copyright Year | 2017 |
| Abstract | In this study, we explored the actions of miR-199a/b-5p during hepatocellular carcinoma (HCC) progression and its potential target genes. Through heatmap miRNA expression analysis of 15 matched HCC tumor and adjacent non-tumor liver tissues from the TCGA database, we detected 19 mRNAs that were upregulated and 13 that were downregulated specifically in HCC. Among these, miR-199 family members were downregulated in HCC tumors and cell lines, as compared to controls. Low miR-199a/b-5p expression was also associated with poor overall survival of HCC patients. miR-199a/b-5p overexpression in HCC cell lines inhibited cell proliferation, migration and invasion, both in vitro and in vivo. In addition, miR199-a/b-5p post-transcriptionally suppressed Rho-associated coiled-coil kinase 1 (ROCK1). This in turn led to inhibition of ROCK1/MLC and PI3K/Akt signaling, which is necessary for HCC proliferation and metastasis. These results indicate that miR-199a/b acts as tumor suppressors in HCC and represent promising therapeutic targets. |
| Starting Page | 67169 |
| Ending Page | 67180 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| DOI | 10.18632/oncotarget.18052 |
| PubMed reference number | 28598808 |
| Alternate Webpage(s) | https://doi.org/10.18632/oncotarget.18052 |
| Volume Number | 8 |
| Journal | Oncotarget |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
