NDLI: Up-regulation of brain nicotinic acetylcholine receptors in the rat during long-term self-administration of nicotine: disproportionate increase of the alpha6 subunit.

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Up-regulation of brain nicotinic acetylcholine receptors in the rat during long-term self-administration of nicotine: disproportionate increase of the alpha6 subunit.

Content Provider	Semantic Scholar
Author	Parker, Steven L. Fu, Yitong McAllen, Kathleen Luo, Jianhong McIntosh, J. Michael Lindstrom, Jon M. Sharp, Burt M.
Copyright Year	2004
Abstract	In male rats continually self-administering nicotine (approximately 1.5 mg free base/kg/day), we found a significant increase of nicotinic acetylcholine receptors (nAChRs) labeled by epibatidine (Epb) in 11 brain areas. A large increase of high-affinity Epb binding sites was apparent in the ventral tegmentum/substantia nigra, nucleus tractus solitarii, nucleus accumbens, thalamus/subthalamus, parietal cortex, hypothalamus, and amygdala. A smaller but significant up-regulation of high-affinity Epb sites was seen in the piriform cortex, hippocampus, caudate/putamen, and cerebellar cortex. The up-regulation of nAChRs, shown by immunoadsorption and Western blotting, involved alpha4, alpha6, and beta2 subunits. As a consequence of long-term self-administration of nicotine, the alpha6 immunoreactive (IR) binding of either labeled Epb or 125I-alpha-conotoxin MII increased to a much greater extent than did alpha4 or beta2 IR binding of Epb. In addition, the beta2 IR binding of Epb was consistently enhanced to a greater extent than was alpha4. These findings may reflect a larger surface membrane retention of alpha6-containing and, to some degree, beta2-containing nAChRs compared with alpha4-containing nAChRs during long-term self-administration of nicotine.
File Format	PDF HTM / HTML
DOI	10.1124/mol.65.3.611
PubMed reference number	14978239
Journal	Medline
Volume Number	65
Issue Number	3
Alternate Webpage(s)	http://molpharm.aspetjournals.org/content/molpharm/65/3/611.full.pdf
Alternate Webpage(s)	https://doi.org/10.1124/mol.65.3.611
Journal	Molecular pharmacology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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