Loading...
Please wait, while we are loading the content...
Similar Documents
HIV infection and antiretroviral therapy: effect on hepatitis C virus quasispecies variability.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shuhart, Margaret C. Bekele, Kirubeal Harrington, Robert D. Mathisen, Terri L. Thomassen, Lisa V. |
| Copyright Year | 2006 |
| Abstract | BACKGROUND Hepatitis C virus (HCV) quasispecies variability has been associated with liver disease progression. The effects of human immunodeficiency virus (HIV) coinfection and highly active antiretroviral therapy (HAART) on HCV quasispecies variability have not been firmly established. METHODS We determined HCV quasispecies complexity and diversity in 69 subjects, 28 of whom were HIV infected, using clonal frequency analysis via heteroduplex mobility analysis of the second envelope gene hypervariable region. Nucleotide sequencing was performed for a small subset of subjects. RESULTS HIV-positive, HAART-naive subjects had significantly lower HCV quasispecies complexity and diversity than did both HIV-negative and HIV-positive HAART-treated subjects. In multivariate analysis, HIV infection predicted decreased complexity (P < .0001) and diversity (P = .001) of HCV quasispecies, whereas HAART predicted increased complexity (P = .013) and diversity (P = .026). For 4 of 6 patients, sequence analysis yielded data supporting the model that positive host pressure drives HCV quasispecies heterogeneity, although data favoring the hypothesis of selective outgrowth of the most fit variants were also observed. CONCLUSION HIV coinfection is associated with decreased HCV quasispecies variability, which appears to be reversed by effective HAART. Although HIV- and HAART-related effects on host immune pressure are likely to play a role in the observed differences in HCV genetic heterogeneity, other mechanisms may be operative. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/jid/193/9/10.1086/502974/2/193-9-1211.pdf?Expires=1491315311&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=aSQtDwlf0PTZ8HLB9PFgELJPTVXHE95ZfQoi0Y~f~3NkvAodwn-9H16HJt7RDFhcr1ZVNGtzxlAk8NXMFzg2He9qphdG1ZwJydxFh8BbJHHLRSVbbX~VddyxazTFopdpDJPVmJ2TChyHkGIfw3J~kR5HwfDHZbBgUlbm9YmsInDhI037RhPtYtLeow3szuA7tyw-EFACL0B~QAYaN6K-rIr5wsGpdHUwPlYHz9YZE1xmC1srkZV-mbX1PuYu5qa9NQxaawoFZhy5PBfm1U5qrDyLapCVqvZzitDuR6-DCx2V1Xq~7EvCR3ONcIfKcb3Y2fWMpDP5YbG8bORrb2-~3w__ |
| Alternate Webpage(s) | https://watermark.silverchair.com/193-9-1211.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAhcwggITBgkqhkiG9w0BBwagggIEMIICAAIBADCCAfkGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMWBnUqJ4L9CzYxEcFAgEQgIIBygOEG7BeU9dIoBS_njj3---NIHIAv1CefRzQgVhYzSx4RRvbetkxVsZCUqZHytUDF55_XlzEXjf8IA40srssdH567iL92my8LO9VntJ71EHyFA12LXAf_9-LsJzuZ0vDVcFyNY6jRX98T7GflnvkIm2hqhdGAC5BGBDjU1suu2XZO8XjTzcnRjQ2zxezY-eZ7jzBK4V3UBjLtjWDjk5hvbvswRie54Ny7_DgxIb-uEcAeg8MpLJbNOJHwL8dwImIJZ1Rjl6GDub624BpZME5h1NnrZMlc3AkLbsXKA5uwdP1oPSu5J6UN6AUVeG1X80RqISvY2ASTBHOPDVYgoI2YVHcLQUWJRix8N7YZ6OTNugGfxYgqetW8vwn-EVER5TJDCwINrSpMa29NJ4eevXi6MrfXH3ZXVLEri2YjDggEHX8Nml8RvNHFNPV1p8IC1xoc23C4DRB63PJpdBhpiqgtCiAF7HJCAh9ERDQHB0YwkqtSORc5zeYus1PCgmfyLE9tdue6fPowxVZk1jBLA7QrzFUuDw_imfFNLFbC4DIIW8WreX7NZg7Dt8qciLaHSsf5V5FN6WZ7Q-P4Kwi6VHuQPDhM4vJDE1yvyFC |
| PubMed reference number | 16586356v1 |
| Volume Number | 193 |
| Issue Number | 9 |
| Journal | The Journal of infectious diseases |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Antiretroviral Therapy, Highly Active Antiretroviral therapy Chemical and Drug Induced Liver Injury Clone Coinfection Genes, env HIV Infections Hepatitis C virus Hereditary Diseases Immunologic Deficiency Syndromes Intrinsic drive Liver diseases Patients Progressive Disease Sequence Analysis Sixty Nine Subgroup |
| Content Type | Text |
| Resource Type | Article |
