Loading...
Please wait, while we are loading the content...
Similar Documents
Dynamics of Human Regulatory T Cells in Lung Lavages of Lung Transplant Recipients
| Content Provider | Scilit |
|---|---|
| Author | Neujahr, David C. Cardona, Adriana C. Ulukpo, Onome Rigby, Mark Pelaez, Andres Ramirez, Allan Gal, Anthony A. Force, Seth D. Lawrence, E. Clinton Kirk, Allan D. Larsen, Christian P. |
| Copyright Year | 2009 |
| Description | Journal: Transplantation Background. Despite advances in the field of lung transplantation, the median survival after lung transplant remains below 5 years. Early rejection is a risk factor for the development of chronic rejection. In animal models of transplant tolerance, regulatory T cells (Tregs) can prevent the establishment of rejection. Methods. This study was designed to explore the dynamics of Tregs focally and systemically in lung transplant recipients. Sequential surveillance bronchoscopy results were available in 51 patients with at least four sequential samples recovered from each patient at defined times posttransplant. In 36 individuals, a complete year of follow-up data for BAL was analyzed. In 33 of these individuals had a complete year of follow-up data for peripheral blood monocyte cell specimens were also analyzed. Lung lavage cells were recovered from each bronchoscopy and corresponding blood draw and subjected to polychromatic flow cytometry. The percentage of CD4 lymphocytes, which expressed the intracellular transcription factor FoxP3 was recorded at each point. At each time point, lung biopsy specimens were scored for rejection. Results. Lung Treg frequency was significantly more variable than blood Treg frequency. Treg frequency in the lung was increased in the aftermath of acute rejection. In contrast, lung Treg frequency declined sequentially in patients demonstrating continued quiescence. Mean BAL Treg level integrated over the first transplant year correlated inversely with the degree of acute cellular rejection. In contrast, blood Treg levels demonstrated no correlation with lung pathology. Conclusions. Lung Tregs increase in the setting of acute cellular rejection, whereas declining levels of BAL Tregs correlates with immunologic quiescence. |
| Related Links | http://europepmc.org/articles/pmc2773802?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773802/pdf |
| Ending Page | 527 |
| Page Count | 7 |
| Starting Page | 521 |
| ISSN | 00411337 |
| e-ISSN | 15346080 |
| DOI | 10.1097/tp.0b013e3181b0e719 |
| Journal | Transplantation |
| Issue Number | 4 |
| Volume Number | 88 |
| Language | English |
| Publisher | Ovid Technologies (Wolters Kluwer Health) |
| Publisher Date | 2009-08-27 |
| Access Restriction | Open |
| Subject Keyword | Journal: Transplantation Respiratory System Lung Transplantation, Regulatory T Cells, Foxp3 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Transplantation |
