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EFFECT OF AMILORIDE ON AGE-DEPENDENT CARDIAC DYSFUNCTION AFTER ISCHEMIA/REPERFUSION IN THE ISOLATED, PERFUSED RAT HEART
| Content Provider | Scilit |
|---|---|
| Author | Linakis, James G. Raymond, Richard M. |
| Copyright Year | 1999 |
| Description | Journal: Shock This study was intended to compare the cardiac consequences of ischemia/reperfusion and amiloride treatment in immature (2–3 wk), juvenile (4–6 wk), and adult (3–5 mo) rats using an isolated, perfused heart model. Male immature, juvenile, and adult rats were anticoagulated and anesthetized. Hearts were harvested and coronary arteries were perfused on a Langendorff apparatus via retrograde perfusion of the aorta at a constant coronary flow (initially determined by perfusing the heart at 50 mmHg perfusion pressure) with oxygenated Krebs-Henseleit-Bicarbonate (KHB) solution. Left ventricular peak systolic (LVPSP) and end diastolic (LVEDP) pressures were measured via a balloon-tipped catheter placed in the left ventricle through the mitral valve. Following a 20–30 min stabilization period, hearts underwent 30 min of normothermic ischemia and were then reperfused with Krebs-Henseleit-Bicarbonate alone for 30 min, or Krebs-Henseleit-Bicarbonate containing 500 μM amiloride for 5 min followed by Krebs-Henseleit-Bicarbonate alone for 25 min (n = 6/age group). Left ventricular generated pressure was calculated (left ventricular peak systolic-left ventricular end diastolic) and used as a measure of ventricular function. All hearts demonstrated a decrease in generated pressure, respectively, from preischemic levels at 15 and 30 min of reperfusion, although this decrease was significantly less for the immature hearts. ischemia/ reperfusion injury was attenuated by amiloride in adult and juvenile hearts, whereas ischemia/reperfusion injury was worsened by amiloride in immature hearts. Although immature hearts were relatively resistant to ischemia/reperfusion injury compared with adult and juvenile hearts, the presence of amiloride during reperfusion resulted in more severe ventricular dysfunction in immature hearts. These data suggest a differential age-dependent mechanism of sarcolemmal ion exchange in response to ischemia/reperfusion. |
| Related Links | http://pdfs.journals.lww.com/shockjournal/1999/03000/EFFECT_OF_AMILORIDE_ON_AGE_DEPENDENT_CARDIAC.11.pdf?token=method|ExpireAbsolute;source|Journals;ttl|1506404372177;payload|mY8D3u1TCCsNvP5E421JYK6N6XICDamxByyYpaNzk7FKjTaa1Yz22MivkHZqjGP4kdS2v0J76WGAnHACH69s21Csk0OpQi3YbjEMdSoz2UhVybFqQxA7lKwSUlA502zQZr96TQRwhVlocEp/sJ586aVbcBFlltKNKo+tbuMfL73hiPqJliudqs17cHeLcLbV/CqjlP3IO0jGHlHQtJWcICDdAyGJMnpi6RlbEJaRheGeh5z5uvqz3FLHgPKVXJzdpGRzmTA0M/Vx2WsqiQOgtRs1/+mU2GdNEPla2Q85JlaFmUV7wwusQfFi7DlzSLnx;hash|aXG8kcQefYMrCSzpl3nFUQ== |
| Ending Page | 223 |
| Page Count | 6 |
| Starting Page | 218 |
| ISSN | 15400514 |
| DOI | 10.1097/00024382-199903000-00011 |
| Journal | Shock |
| Issue Number | 3 |
| Volume Number | 11 |
| Language | English |
| Publisher | Ovid Technologies (Wolters Kluwer Health) |
| Publisher Date | 1999-03-01 |
| Access Restriction | Open |
| Subject Keyword | Peripheral Vascular Disease Ischemia/reperfusion |
| Content Type | Text |
| Resource Type | Article |
| Subject | Emergency Medicine Critical Care and Intensive Care Medicine |
