Loading...
Please wait, while we are loading the content...
Similar Documents
CALCIUM BLOCKADE REDUCES RENAL APOPTOSIS DURING ISCHEMIA REPERFUSION
| Content Provider | Scilit |
|---|---|
| Author | Raafat, Aml M. Murray, Mary T. McGuire, Timothy Defrain, Michael Franko, Alexander P. Zafar, Rasheeda S. Palmer, Kenneth Diebel, Lawrence Dulchavsky, Scott A. |
| Copyright Year | 1997 |
| Description | Journal: Shock Apoptosis is well described in invertebrates and recently documented in mammals. The prevalence and pathophysiology of mammalian apoptosis is unknown and may have clinical ramifications. The aim of this study is to investigate the apoptotic response during kidney ischemia-reperfusion (I/R) injury. Kidney I/R was initiated in anesthetized rats by occlusion of the renal pedicle for 45 min with or without pretreatment with .2 mg/kg verapamil: control animals received sham exposure. Flow was re-established after ischemia and the animals were allowed to recover for 24 h. Bilateral kidneys were harvested for DNA electrophoresis, Western analysis for p53, Northern analysis for c-myc expression, and light and electron microscopic analysis. Kidney I/R caused characteristic DNA laddering in the clamped kidney, and less extensive laddering was seen in the contralateral kidney. Light and electron microscopic analysis confirmed apoptotic morphology in the reperfused tissues. Verapamil pretreatment completely abolished DNA laddering and attenuated the microscopic evidence of apoptosis. p53 levels were increased by I/R in the ischemic kidney and moderately increased in the contralateral organ, c-myc mRNA levels were increased by the I/R insult. Kidney I/R injury may induce global apoptosis, which seems to be associated with an alteration in calcium homeostasis. The increase in p53 and c-myc mRNA levels seen with I/R may facilitate apoptosis. Calcium modulation seems to reduce apoptosis during I/R and may have therapeutic implications. |
| Related Links | http://pdfs.journals.lww.com/shockjournal/1997/09000/CALCIUM_BLOCKADE_REDUCES_RENAL_APOPTOSIS_DURING.6.pdf?token=method|ExpireAbsolute;source|Journals;ttl|1503216881566;payload|mY8D3u1TCCsNvP5E421JYK6N6XICDamxByyYpaNzk7FKjTaa1Yz22MivkHZqjGP4kdS2v0J76WGAnHACH69s21Csk0OpQi3YbjEMdSoz2UhVybFqQxA7lKwSUlA502zQZr96TQRwhVlocEp/sJ586aVbcBFlltKNKo+tbuMfL73hiPqJliudqs17cHeLcLbV/CqjlP3IO0jGHlHQtJWcICDdAyGJMnpi6RlbEJaRheGeh5z5uvqz3FLHgPKVXJzdpGRzmTA0M/Vx2WsqiQOgtckiK/at61LGv/4lGZy6gdsJH27jkELiw2zN4sBlHJui;hash|NlJu4lXGovN+NqJA4sX4ng== |
| Ending Page | 192 |
| Page Count | 7 |
| Starting Page | 186 |
| ISSN | 15400514 |
| DOI | 10.1097/00024382-199709000-00006 |
| Journal | Shock |
| Issue Number | 3 |
| Volume Number | 8 |
| Language | English |
| Publisher | Ovid Technologies (Wolters Kluwer Health) |
| Publisher Date | 1997-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Shock Peripheral Vascular Disease Kidney I/r |
| Content Type | Text |
| Resource Type | Article |
| Subject | Emergency Medicine Critical Care and Intensive Care Medicine |
