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α1AMP-Activated Protein Kinase Preserves Endothelial Function During Chronic Angiotensin II Treatment by Limiting Nox2 Upregulation
| Content Provider | Scilit |
|---|---|
| Author | Schuhmacher, Swenja Foretz, Marc Knorr, Maike Jansen, Thomas Hortmann, Marcus Wenzel, Philip Oelze, Matthias Kleschyov, Andrei L. Daiber, Andreas Keaney Jr, John F. Wegener, Gerhard Lackner, Karl Münzel, Thomas Viollet, Benoit Schulz, Eberhard |
| Copyright Year | 2011 |
| Description | Journal: Arteriosclerosis, thrombosis, and vascular biology Objective—: Besides its well-described metabolic effects, vascular AMP-activated protein kinase (AMPK) can activate endothelial NO synthase, promotes angiogenesis, and limits endothelial cell apoptosis. The current study was designed to study the effects of α1AMPK deletion during vascular disease in vivo. Methods and Results—: Chronic angiotensin II infusion at low subpressor doses caused a mild endothelial dysfunction that was significantly aggravated in α1AMPK-knockout mice. Unexpectedly, this endothelial dysfunction was not associated with decreased NO content, because NO levels measured by serum nitrite or electron paramagnetic resonance were even increased. However, because of parallel superoxide production, NO was consumed under production of peroxynitrite in angiotensin II–treated α1AMPK-knockout mice, associated with NADPH oxidase activation and Nox2 upregulation. As Nox2 is also a component of phagocyte NADPH oxidases, we found a vascular upregulation of several proinflammatory markers, including inducible NO synthase, vascular cell adhesion molecule-1, and cyclooxygenase-2. Cotreatment with the NADPH oxidase inhibitor apocynin was able to prevent vascular inflammation and also partially restored endothelial function in α1AMPK-knockout mice. Conclusion—: Our data indicate that in vivo α1AMPK deletion leads to Nox2 upregulation, resulting in endothelial dysfunction and vascular inflammation. This implicates basal AMPK activity as a protective, redox-regulating element in vascular homeostasis. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066307/pdf https://www.ahajournals.org/doi/pdf/10.1161/ATVBAHA.110.219543 |
| Ending Page | 566 |
| Page Count | 7 |
| Starting Page | 560 |
| ISSN | 10795642 |
| e-ISSN | 15244636 |
| DOI | 10.1161/atvbaha.110.219543 |
| Journal | Arteriosclerosis, thrombosis, and vascular biology |
| Issue Number | 3 |
| Volume Number | 31 |
| Language | English |
| Publisher | Ovid Technologies (Wolters Kluwer Health) |
| Publisher Date | 2011-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Arteriosclerosis, thrombosis, and vascular biology Peripheral Vascular Disease Nitric Oxide Angiotensin Ii Reactive Oxygen Species Vascular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cardiology and Cardiovascular Medicine |
