NDLI: Amplification ofrfbEandfliCGenes by Polymerase Chain Reaction for Identification and Detection ofSalmonellaSerovar Enteritidis, Dublin and Gallinarum-Pullorum

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Amplification ofrfbEandfliCGenes by Polymerase Chain Reaction for Identification and Detection ofSalmonellaSerovar Enteritidis, Dublin and Gallinarum-Pullorum

Content Provider	Scilit
Author	Itoh, Yoshihiro Hirose, Kenji Miyake, Masaki Khan, Abdul Q. Hashimoto, Yasuhiro Ezaki, Takayuki
Copyright Year	1997
Description	Journal: Microbiology and Immunology Polymerase chain reaction (PCR) primers for O9 antigen (rfbE) and phase 1 flagellin antigen (fliC) were designed for the rapid identification and detection of Salmonella serovar Enteritidis and Dublin. The rfbE primer pairs selectively amplified the rfbE region of group O9 Salmonella serovars. The fliC primer pairs amplified the DNAs of g,m and g,p-type flagellar antigen; Salmonella serovar Enteritidis, Dublin, and Essen. However, DNA from flagellar-negative Salmonella serovar Gallinarum-Pullorum was also amplified. The sensitivity of PCR primer pairs was 10(4) CFU/assay by boiled DNA preparation and 10(2) CFU/assay by proteinase K-treated DNA preparation.
Ending Page	794
Starting Page	791
ISSN	2573508X
e-ISSN	13480421
DOI	10.1111/j.1348-0421.1997.tb01928.x
Journal	Microbiology and Immunology
Issue Number	10
Volume Number	41
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1997-10-01
Access Restriction	Open
Subject Keyword	Journal: Microbiology and Immunology Metallurgy and Metallurgical Engineering Rfbe Gene Flic Gene Salmonella Serovar Enteritidis Salmonella Serovar Dublin Salmonella Serovar Gallinarum‐pullorum
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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