NDLI: Detection of Various Variant Verotoxin Genes inEscherichia coliby Polymerase Chain Reaction

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Detection of Various Variant Verotoxin Genes inEscherichia coliby Polymerase Chain Reaction

Content Provider	Scilit
Author	Lin, Zaw Kurazono, Hisao Yamasaki, Shinji Takeda, Yoshifumi
Copyright Year	1993
Description	Journal: Microbiology and Immunology We constructed common primers for the polymerase chain reaction to detect the genes for various Verotoxins reported, that is, VT1 (or SLT-I), VT2 (or SLT-II), VT2vha, VT2vhb, SLT-IIv (or VT2vp1, VTe) and SLT-IIva (or VT2vp2). A total of 80 Verocytotoxin-producing Escherichia coli strains isolated from humans, domestic animals and meats gave a positive result by PCR with the designed common primers. Digestion by restriction endonucleases BglII and EcoT14I of the amplicon of the VT2vp2 gene gave specific bands of the expected sizes, but not of the amplicons of other VT genes, suggesting a possible method for identification of the VT2vp2 gene. Application of the PCR with the designed primers in diagnostic and epidemiological studies on VTEC infection is also discussed.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.1993.tb01675.x/pdf
Ending Page	548
Page Count	6
Starting Page	543
ISSN	03855600
e-ISSN	13480421
DOI	10.1111/j.1348-0421.1993.tb01675.x
Journal	Microbiology and Immunology
Issue Number	7
Volume Number	37
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1993-07-01
Access Restriction	Open
Subject Keyword	Journal: Microbiology and Immunology Metallurgy and Metallurgical Engineering Verotoxin Gene Polymerase Chain Reaction (pcr)
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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