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Enabling $hyperpolarized^{129}$Xe MR spectroscopy and imaging of pulmonary gas transfer to the red blood cells in transgenic mice expressing human hemoglobin
| Content Provider | Scilit |
|---|---|
| Author | Freeman, Matthew S. Cleveland, Zackary I. Qi, Yi Driehuys, Bastiaan |
| Copyright Year | 2013 |
| Description | Journal: Magnetic Resonance in Medicine Purpose Hyperpolarized (HP)$ ^{129}$Xe gas in the alveoli can be detected separately from$ ^{129}$Xe dissolved in pulmonary barrier tissues (blood plasma and parenchyma) and red blood cells (RBCs) of humans, allowing this isotope to probe impaired gas uptake. Unfortunately, mice, which are favored as lung disease models, do not display a unique RBC resonance, thus limiting the preclinical utility of$ ^{129}$Xe MR. Here we overcome this limitation using a commercially available strain of transgenic mice that exclusively expresses human hemoglobin. Methods Dynamic HP$ ^{129}$Xe MR spectroscopy, and three‐dimensional radial MRI of gaseous and dissolved$ ^{129}$Xe were performed in both wild‐type (C57BL/6) and transgenic mice. Results Unlike wild‐type animals, transgenic mice displayed two dissolved$ ^{129}$Xe NMR peaks at 198 and 217 ppm, corresponding to$ ^{129}$Xe dissolved in barrier tissues and RBCs, respectively. Moreover, signal from these resonances could be imaged separately, using a 1‐point variant of the Dixon technique. Conclusion It is now possible to examine the dynamics and spatial distribution of pulmonary gas uptake by the RBCs of mice using HP$ ^{129}$Xe MR spectroscopy and imaging. When combined with ventilation imaging, this ability will enable translational “mouse‐to‐human” studies of impaired gas exchange in a variety of pulmonary diseases. Magn Reson Med 70:1192–1199, 2013. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011488/pdf |
| Ending Page | 1199 |
| Page Count | 8 |
| Starting Page | 1192 |
| e-ISSN | 15222594 |
| DOI | 10.1002/mrm.24915 |
| Journal | Magnetic Resonance in Medicine |
| Issue Number | 5 |
| Volume Number | 70 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2013-09-04 |
| Access Restriction | Open |
| Subject Keyword | Journal: Magnetic Resonance in Medicine Radiology, Nuclear Medicine and Imaging Hyperpolarized 129xe Gas Exchange Mouse Model Red Blood Cell Interstitial Lung Disease Chemical Shift Saturation Recovery |
| Content Type | Text |
