NDLI: A rapid protocol for isolation of RNA from mycobacteria

Content Provider	Scilit
Author	Rajagopalan, M. Boggaram, V. Madiraju, M. V. V. S.
Copyright Year	1995
Description	Journal: Letters in applied microbiology Isolation of total cellular RNA from members of mycobacteria has been a labour-intensive task involving large volumes of cells, multiple extractions of cell lysates with phenol-chloroform followed by caesium chloride centrifugation. A simple and rapid procedure is reported for isolation of RNA from mycobacteria using as few as 1 x 10(7) cells. The RNA thus isolated when analysed on ethidium bromide gels contained 16S and 23S RNA as major species. Further, the RNA was used for amplification of an internal segment of hsp65 gene by reverse transcription followed by PCR.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.1995.tb00995.x/pdf
Ending Page	17
Page Count	4
Starting Page	14
e-ISSN	1472765X
DOI	10.1111/j.1472-765x.1995.tb00995.x
Journal	Letters in applied microbiology
Issue Number	1
Volume Number	21
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1995-07-01
Access Restriction	Open
Subject Keyword	Journal: Letters in applied microbiology Analytical Chemistry Rapid Protocol Protocol for Isolation Isolation of Rna From Mycobacteria
Content Type	Text
Resource Type	Article

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A rapid protocol for isolation of RNA from mycobacteria