NDLI: Rapid isolation and purification of lactococcal bacteriophage DNA without the use of caesium chloride gradients

Content Provider	Scilit
Author	Brown, J. C. S. Ward, L. J. H. Davey, G. P.
Copyright Year	1994
Description	Journal: Letters in applied microbiology Intact bacteriophage of Lactococcus lactis were recovered from small volumes of lysate by centrifugation at 15,000 g without precipitation with polyethylene glycol and sodium chloride, or ultracentrifugation in a caesium chloride gradient. DNA was then extracted and purified by standard protocols. This DNA was readily digested with restriction endonucleases and used successfully in hybridization experiments.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.1994.tb00872.x/pdf
Ending Page	293
Page Count	2
Starting Page	292
e-ISSN	1472765X
DOI	10.1111/j.1472-765x.1994.tb00872.x
Journal	Letters in applied microbiology
Issue Number	5
Volume Number	18
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1994-05-01
Access Restriction	Open
Subject Keyword	Journal: Letters in applied microbiology Analytical Chemistry Lactococcal Bacteriophage Rapid Isolation Isolation and Purification Chloride Gradients Caesium Chloride
Content Type	Text
Resource Type	Article

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Rapid isolation and purification of lactococcal bacteriophage DNA without the use of caesium chloride gradients