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Studies on the Purification and Properties of a 6.8-S DNA Polymerase Activity Found in Calf-Thymus DNA Polymerase-alpha Fraction
| Content Provider | Scilit |
|---|---|
| Author | Hesslewood, Ian P. Holmes, Andrew M. Wakeling, William F. Johnston, Irving R. |
| Copyright Year | 1978 |
| Description | Journal: Journal of Biological Inorganic Chemistry The heterogeneity of calf thymus DNA polymerase-alpha has been further investigated. In particular, an enzyme (enzyme D) which exhibits higher activity on poly(dA) . (dT)10 (A:T = 20:1) compared with that on activated DNA, has been further purified and its properties compared with two other activities of the DNA polymerase-alpha fraction (enzymes A1 and C) which do not show a preference for poly(dA) . (dT)10 over activated DNA. As with A1 and C, enzyme D was shown to have many of the characteristic properties of DNA polymerase-alpha in that it is an acidic protein as judged by its binding to DEAE-cellulose, has a molecular weight of about 140000, does not use a poly (A) . (dT)10 template-initiator complex and is inhibited by N-ethylmaleimide. It exhibits anomalous gel filtration behaviour on Sepharose 6B and it binds relatively weakly to DNA-cellulose compared with DNA polymerase-beta. The extreme sensitivity of enzyme D to inhibtion by N-ethylmaleimide distinguishes it from A1 and C, as does its elution position from a DEAE-cellulose column. On the other hand enzymes C and D are readily inactivated by heating at 45 degrees C unlike enzyme A1. The possible interrelationships of the multiple activities of calf thymus DNA polymerase-alpha are discussed. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1978.tb12148.x/pdf |
| Ending Page | 131 |
| Page Count | 9 |
| Starting Page | 123 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1978.tb12148.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 84 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1978-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemical Research Analytical Chemistry Dna Polymerase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
