Loading...
Please wait, while we are loading the content...
Similar Documents
Characterization of DNA Kinase from Calf Thymus
| Content Provider | Scilit |
|---|---|
| Author | Tamura, Sanae Teraoka, Hirobumi Tsukada, Kinji |
| Copyright Year | 1981 |
| Description | Journal: Journal of Biological Inorganic Chemistry DNA kinase has been purified to homogeneity from calf thymus. The purified enzyme, with a specific activity of 16.7 units/mg protein at 25° C, exhibited a sharp pH/activity curve with a pH optimum at 5.5 and low activity at alkaline pH. The molecular weight of the enzyme was estimated by dodecylsulfate/polyacrylamide gel electrophoresis to be 5.4 × $10^{4}$. The enzyme has a sedimentation coefficient of 4.0 S. An apparent molecular weight of 5.6 × $10^{4}$ and a Stokes' radius of 3.3 nm were estimated by gel-filtration on Sephadex G-100. The enzyme phosphorylates neither yeast RNA nor poly(A) instead of DNA. Compared with rat liver DNA kinase, calf thymus DNA kinase is relatively resistant to the inhibition by sulfate $(K_{i}$= 7 mM) and pyrophosphate $(K_{i}$= 5 mM). The enzyme activity is markedly stimulated by polyamines at the sub-optimal concentration of $Mg^{2+}$ but not by monovalent cations. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1981.tb06222.x |
| Ending Page | 453 |
| Page Count | 5 |
| Starting Page | 449 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1981.tb06222.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 3 |
| Volume Number | 115 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1981-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemical Research Thymus Dna Kinase Dna Kinase From Calf Thymus |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
