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Phosphofructokinase: Complete Amino-Acid Sequence of the Enzyme from Bacillus stearothermophilus
| Content Provider | Scilit |
|---|---|
| Author | Kolb, Edith Hudson, Peter J. Harris, J. Ieuan |
| Copyright Year | 1980 |
| Description | Journal: Journal of Biological Inorganic Chemistry The entire amino acid sequence of the protein subunit of phosphofructokinase from Bacillus stearothermophilus has been established mainly by sequence analysis of cyanogen bromide fragments and of peptides derived from these fragments by further digestion with proteolytic enzymes. Overlaps of the cyanogen bromide fragments as well as peptide sequences necessary to complement and to confirm tentative assignments within the larger peptide fragments were obtained from the sequences of selected peptides isolated from tryptic and chymotryptic digests of the intact $S-[^{14}$C]-carboxymethylated protein. Sequence information was also provided by automated sequence analysis of the intact protein subunit and of some of the larger peptide fragments. The sequence is as follows: |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1980.tb04754.x |
| Ending Page | 597 |
| Page Count | 11 |
| Starting Page | 587 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1980.tb04754.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 108 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1980-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemical Research Protein Subunit Bacillus Stearothermophilus Bromide Fragments Acid Sequence Cyanogen Bromide Amino Acid Larger Peptide Sequence Analysis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
