Loading...
Please wait, while we are loading the content...
Similar Documents
DMSO‐ and Serum‐Free Cryopreservation of Wharton's Jelly Tissue Isolated From Human Umbilical Cord
| Content Provider | Scilit |
|---|---|
| Author | Shivakumar, Sharath Belame Bharti, Dinesh Subbarao, Raghavendra Baregundi Jang, Si‐Jung Park, Ji‐Sung Ullah, Imran Byun, June‐Ho Park, Bong‐Wook Rho, Gyu‐Jin |
| Copyright Year | 2016 |
| Description | Journal: Journal of Cellular Biochemistry The facile nature of mesenchymal stem cell (MSC) acquisition in relatively large numbers has made Wharton's jelly (WJ) tissue an alternative source of MSCs for regenerative medicine. However, freezing of such tissue using dimethyl sulfoxide (DMSO) for future use impedes its clinical utility. In this study, we compared the effect of two different cryoprotectants (DMSO and cocktail solution) on post‐thaw cell behavior upon freezing of WJ tissue following two different freezing protocols (Conventional [−1°C/min] and programmed). The programmed method showed higher cell survival rate compared to conventional method of freezing. Further, cocktail solution showed better cryoprotection than DMSO. Post‐thaw growth characteristics and stem cell behavior of Wharton's jelly mesenchymal stem cells (WJMSCs) from WJ tissue cryopreserved with a cocktail solution in conjunction with programmed method (Prog‐Cock) were comparable with WJMSCs from fresh WJ tissue. They preserved their expression of surface markers, pluripotent factors, and successfully differentiated in vitro into osteocytes, adipocytes, chondrocytes, and hepatocytes. They also produced lesser annexin‐V‐positive cells compared to cells from WJ tissue stored using cocktail solution in conjunction with the conventional method (Conv‐Cock). Real‐time PCR and Western blot analysis of post‐thaw WJMSCs from Conv‐Cock group showed significantly increased expression of pro‐apoptotic factors (BAX, p53, and p21) and reduced expression of anti‐apoptotic factor (BCL2) compared to WJMSCs from the fresh and Prog‐Cock group. Therefore, we conclude that freezing of fresh WJ tissue using cocktail solution in conjunction with programmed freezing method allows for an efficient WJ tissue banking for future MSC‐based regenerative therapies. J. Cell. Biochem. 117: 2397–2412, 2016. © 2016 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals, Inc. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094545/pdf |
| Ending Page | 2412 |
| Page Count | 16 |
| Starting Page | 2397 |
| e-ISSN | 10974644 |
| DOI | 10.1002/jcb.25563 |
| Journal | Journal of Cellular Biochemistry |
| Issue Number | 10 |
| Volume Number | 117 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2016-06-23 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Cellular Biochemistry Cell Tissue Engineering Biomedical Engineering Cell Survival Rate Cocktail Solution Wharton's Jelly Mesenchymal Stem Cells Wharton's Jelly Tissue |
| Content Type | Text |
| Resource Type | Article |
