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The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation
| Content Provider | Scilit |
|---|---|
| Author | Abou-ElNaga, Amoura El-Chennawi, Farha Kamel, Samar Ibrahim Mutawa, Ghada |
| Copyright Year | 2020 |
| Description | Background: The new therapeutic strategy of managing cardiac diseases is based on cell therapy; it highly suggests the use of multipotent mesenchymal stem/stromal cells (MSCs). MSCs widely used in researches are known to be isolated from bone marrow. However, this research seeks to use a human umbilical cord (HUC) as an alternative source of MSCs. Since HUC Wharton's jelly (WJ)-isolated MSCs originate as fetal tissue they are highly preferable for their potential advantages over other adult tissues. Methods: The researchers used enzymatic digestion to establish a primary HUC-WJ-isolated MSC line. Then, flow cytometry was used to characterize MSCs and hematopoietic stem cells (HSCs) markers' expression. In addition, the cardiac differentiation capacity of HUC-WJ-isolated MSCs in vitro was investigated by two protocols. Protocol-1 necessitates the dependence on merely 5-azacytidine (5-Aza), whereas in protocol-2, 5-Aza was supported by basic fibroblast growth factor (BFGF). The comparative study between the two protocols was applied by inspecting the ultrastructure of differentiated cells, measuring RT-PCR mRNA cardiac markers and the quantitative detection of cardiac proteins. Results: HUC-WJ isolated MSCs were expressed by $CD90^{+ve}$, $CD105^{+ve}$, $CD106^{+ve}$, $CD45^{−ve}$, and $CD146^{−ve}$. Remarkable TNNT1, NKX2.5, and Desmin mRNA expression and higher quantitative LDH and cTnI were detected by applying protocol-2. This same protocol-2 induced cardiac morphological features that were revealed by identifying cardiomyocyte-like cells and typical sarcomeres. Conclusion: HUC-WJ is proved to be an ethical and effective source of MSCs induced cardiac differentiation, whereas BFGF supports 5-Aza in MSCs-cardiomyocytes differentiation. |
| Related Links | https://www.dovepress.com/getfile.php?fileID=63489 |
| ISSN | 11786957 |
| e-ISSN | 11786957 |
| DOI | 10.2147/sccaa.s253108 |
| Journal | Stem Cells and Cloning: Advances and Applications |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-11-01 |
| Access Restriction | Open |
| Subject Keyword | Cell Tissue Engineering Integrative and Complementary Medicine 5-azacytidine Wharton's Jelly Cardiomyocytes Fibroblast Growth Factor Human Umbilical Cord Mesenchymal Stem/stromal Cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
