Loading...
Please wait, while we are loading the content...
Similar Documents
Characterization and Subcellular Localization of l-[3H]Carnitine Binding Sites in Rat Brain
| Content Provider | Scilit |
|---|---|
| Author | Vesci, Loredana Tobia, Patrizia Corsico, Nerina Martelli, Edoardo Arrigoni Arduini, Arduino |
| Copyright Year | 2002 |
| Description | Journal: Journal of Neurochemistry In the present study, we investigated the existence of a binding site for L-carnitine in the rat brain. In crude synaptic membranes, L-[3H]carnitine bound with relatively high affinity (KD = 281 nM) and in a saturable manner to a finite number (apparent Bmax value = 7.3 pmol/mg of protein) of binding sites. Binding was reversible and dependent on protein concentration, pH, ionic strength, and temperature. Kinetic studies revealed a Koff of 0.018 min-1 and a Kon of 0.187 x 10(-3) min-1 nM-1. Binding was highest in spinal cord, followed by medulla oblongata-pons > or = corpus striatum > or = cerebellum = cerebral cortex = hippocampus = hypothalamus = olfactory bulb. L-[3H]Carnitine binding was stereoselective for the L-isomers of carnitine, propionylcarnitine, and acetylcarnitine. The most potent inhibitor of L-[3H]carnitine binding was L-carnitine followed by propionyl-L-carnitine. Acetyl-L-carnitine and isobutyryl-L-carnitine showed an affinity approximately 500-fold lower than that obtained for L-carnitine. The precursor gamma-butyrobetaine had negligible activity at 0.1 mM. L-Carnitine binding to rat crude synaptic membrane preparation was not inhibited by neurotransmitters (GABA, glycine, glutamate, aspartate, acetycholine, dopamine, norepinephrine, epinephrine, 5-hydroxytryptamine, histamine) at a final concentration of 0.1 mM. In addition, the binding of these neuroactive compounds to their receptors was not influenced by the presence of 0.1 mM L-carnitine.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.1995.64062783.x/pdf |
| Ending Page | 2791 |
| Page Count | 9 |
| Starting Page | 2783 |
| e-ISSN | 14714159 |
| DOI | 10.1046/j.1471-4159.1995.64062783.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 6 |
| Volume Number | 64 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2002-11-23 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Synaptic Membranes Carnitine Binding Crude Synaptic Membrane |
| Content Type | Text |
| Resource Type | Article |
