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Characterization of ?-Aminobutyric Acid Binding Sites on Crude Synaptic Membranes
| Content Provider | Scilit |
|---|---|
| Author | Kurioka, Susumu Hayata, Hideo Matsuda, Makoto |
| Copyright Year | 1981 |
| Description | Journal: Journal of Neurochemistry [3H]GABA binding to crude synaptic membranes of rat brain was studied in an attempt to identify GABA binding to its synaptic receptor in the presence of Na+. Membrane vesicles prepared from crude synaptic membrane fractions were useful as a tool to differentiate synaptic GABA receptors from GABA uptake sites. The crude synaptic membranes treated with Triton X-100 [membranes (TX)] involved two classes of GABA binding sites (KD = 38.7 and 78.0 nM) in the absence of Na+, but the high-affinity sites disappeared in the presence of Na+ and a single class of GABA binding sites (KD = 75.0 nM) was detected. The failure to detect an active uptake of [3H]GABA into the vesicles prepared from membranes (TX) suggests that the [3H]GABA binding in the presence of Na+ was related to synaptic GABA receptors. It is probable that Na+ could mask the presence of the high-affinity class of GABA receptor. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1981.tb00453.x/pdf |
| Ending Page | 288 |
| Page Count | 6 |
| Starting Page | 283 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1981.tb00453.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 2 |
| Volume Number | 37 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1981-08-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Γ‐aminobutyric Acid Binding Rat Brain Membranes Membrane Vesicles Synaptic Receptor |
| Content Type | Text |
| Resource Type | Article |
