Loading...
Please wait, while we are loading the content...
Genomic and mutational profiling of ductal carcinomas in situ and matched adjacent invasive breast cancers reveals intra-tumour genetic heterogeneity and clonal selection
| Content Provider | Scilit |
|---|---|
| Author | Hernandez, Lucia Wilkerson, Paul M. Lambros, Maryou B. Campion-Flora, Adriana Rodrigues, Daniel Nava Gauthier, Arnaud Cabral, Cecilia Pawar, Vidya Mackay, Alan A'Hern, Roger Marchiò, Caterina Palacios, Jose Natrajan, Rachael Weigelt, Britta Reis-Filho, Jorge S. |
| Copyright Year | 2012 |
| Description | Journal: The Journal of Pathology The mechanisms underlying the progression from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) of the breast are yet to be fully elucidated. Several hypotheses have been put forward to explain the progression from DCIS to IDC, including the selection of a subpopulation of cancer cells with specific genetic aberrations, and the acquisition of new genetic aberrations or non-genetic mechanisms mediated by the tumour microenvironment. To determine whether synchronously diagnosed ipsilateral DCI and IDCs have modal populations with distinct repertoires of gene copy number aberrations and mutations in common oncogenes, matched frozen samples of DCIS and IDC were retrieved from 13 patients and subjected to microarray-based comparative genomic hybridization (aCGH) and Sequenom MassARRAY (Oncocarta v 1.0 panel). Fluorescence in situ hybridization and Sanger sequencing were employed to validate the aCGH and Sequenom findings, respectively. Although the genomic profiles of matched DCI and IDCs were similar, in three of 13 matched pairs amplification of distinct loci (ie 1q41, 2q24.2, 6q22.31, 7q11.21, 8q21.2 and 9p13.3) was either restricted to, or more prevalent in, the modal population of cancer cells of one of the components. Sequenom MassARRAY identified PIK3CA mutations restricted to the DCIS component in two cases, and in a third case the frequency of the PIK3CA mutant allele reduced from 49% in the DCIS to 25% in the IDC component. Despite the genomic similarities between synchronous DCIS and IDC, our data provide strong circumstantial evidence to suggest that in some cases the progression from DCIS to IDC is driven by the selection of non-modal clones that harbour a specific repertoire of genetic aberrations. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975517/pdf |
| Ending Page | 52 |
| Page Count | 11 |
| Starting Page | 42 |
| e-ISSN | 10969896 |
| DOI | 10.1002/path.3990 |
| Journal | The Journal of Pathology |
| Issue Number | 1 |
| Volume Number | 227 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2012-01-17 |
| Access Restriction | Open |
| Subject Keyword | Journal: The Journal of Pathology Microarray-based Comparative Genomic Hybridization Fluorescence in Situ Hybridization Sequenom Massarray |
| Content Type | Text |
| Resource Type | Article |
