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Partial Purification and Properties of the Inhibitors of Na,K-ATPase and Ouabain-Binding in Bovine Central Nervous System
| Content Provider | Scilit |
|---|---|
| Author | Akagawa, Kimio Hara, Naoyuki Tsukada, Yasuzo |
| Copyright Year | 1984 |
| Description | Journal: Journal of Neurochemistry Endogenous inhibitors of Na,K-ATPase and ouabain-binding were partially purified from bovine central nervous system, and some of their properties were studied. They were eluted as low-molecular-weight fractions by gel filtration. They could be adsorbed by both Amberlite IR 120 and Amberlite IRA 400 at acidic and basic pH, respectively, indicating that they could act as both anions and cations at different pH. These inhibitors of ouabain-binding appeared to affect specific binding of ouabin, and Scatchard plot analysis showed that the inhibition was competitive, suggesting that they could bind to the same site as ouabain, presumably to Na,K-ATPase itself. The inhibitory activities were heat stable, but charring inactivated them completely. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1984.tb02749.x/pdf |
| Ending Page | 780 |
| Page Count | 6 |
| Starting Page | 775 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1984.tb02749.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 3 |
| Volume Number | 42 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1984-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry |
| Content Type | Text |
| Resource Type | Article |
