Loading...
Please wait, while we are loading the content...
Characterization of "High-Affinity"[3H]Ouabain Binding in the Rat Central Nervous System
| Content Provider | Scilit |
|---|---|
| Author | Hauger, R. Luu, H. M. D. Meyer, D. K. Goodwin, F. K. Paul, S. M. |
| Copyright Year | 1985 |
| Description | Journal: Journal of Neurochemistry The characteristics of [3H]ouabain binding were examined in various areas of rat brain. In the striatum, Scatchard analysis revealed a single class of "high-affinity" binding sites with an apparent binding affinity (KD) of 10.4 +/- 0.9 nM and an estimated binding capacity (Bmax) of 7.6 +/- 1.9 pmol/mg protein. Similar monophasic Scatchard plots were found in the brainstem, cerebellum, hypothalamus, and frontal cerebral cortex. [3H]Ouabain binding to rat brain was sodium- and ATP-dependent and strongly inhibited by potassium. Proscillariden A was the most potent cardiac glycoside tested in inhibiting specific [3H]ouabain binding to brain membranes, and the rank order of inhibitory potencies for a series of cardiac glycosides was similar to that previously reported for inhibition of heart Na,K-ATPase. To assess whether the high-affinity binding sites for [3H]ouabain were localized to neuronal or nonneuronal membranes, the effect of discrete kainic acid lesions on striatal [3H]ouabain binding was examined. Kainic acid lesions of the striatum reduced [3H]ouabain binding to striatal homogenates by 79.6 +/- 1.6%. This suggests that the "high-affinity" [3H]ouabain binding sites measured in our experiments are localized to neuronal elements. Thus, the high-affinity binding of [3H]ouabain to brain membranes may selectively label a neuronal form or conformation of Na,K-ATPase. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1985.tb07158.x/pdf |
| Ending Page | 1715 |
| Page Count | 7 |
| Starting Page | 1709 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1985.tb07158.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 6 |
| Volume Number | 44 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1985-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Biochemistry and Molecular Biology “high‐affinity”[3h]ouabain Binding |
| Content Type | Text |
| Resource Type | Article |
