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Excitatory Amino Acid Receptor Potency and Subclass Specificity of Sulfur-Containing Amino Acids
| Content Provider | Scilit |
|---|---|
| Author | Pullan, L. M. Olney, J. W. Price, M. T. Compton, R. P. Hood, W. F. Michel, J. Monahan, J. B. |
| Copyright Year | 1987 |
| Description | Journal: Journal of Neurochemistry The sulfur-containing amino acids, L- and D-cysteate, L-cysteine, L- and D-cysteine sulfinate, L- and D-cysteine-S-sulfate, L-cystine, L- and D-homocysteate, L- and D-homocysteine sulfinate, L-homocysteine, L-serine-O-sulfate, and taurine were tested in two excitatory amino acid receptor functional assays and in receptor binding assays designed to label specifically the AA1/N-methyl-D-aspartate (NMDA), AA2/quisqualate, and AA3/kainate receptor recognition sites, as well as a CaCl2-dependent L-2-amino-4-phosphonobutanoate site, and a putative glutamate uptake site. Agonist efficacies were determined by chick retinal excitotoxicity and stimulated sodium efflux from rat brain slices. D-Homocysteine sulfinate, L-homocysteate, and L-serine-O-sulfate had affinities most selective for the NMDA binding site, whereas the binding affinities of D-cysteate, D-cysteine sulfinate, D-homocysteate, and L-homocysteine sulfinate were less selective. However, the correlation of agonist activity sensitive to blockade by D-2-amino-7-phosphonoheptanoate or D-2-amino-5-phosphonopentanoate in the functional assays with affinity in the NMDA binding assay (r = 0.87, p less than 0.005 and r = 0.98, p less than 0.005 for excitotoxicity and sodium efflux, respectively) allows characterization of these sulfur-containing amino acids as acting at NMDA subclass receptors. L-Homocysteate, which has been found in the brain, and L-serine-O-sulfate are selective agonists and could serve as endogenous neurotransmitters at the NMDA receptor. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1987.tb10024.x/pdf |
| Ending Page | 1307 |
| Page Count | 7 |
| Starting Page | 1301 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1987.tb10024.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 4 |
| Volume Number | 49 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1987-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Sulfur‐containing Excitatory Amino Acids N‐methyl‐d‐aspartate |
| Content Type | Text |
| Resource Type | Article |
