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Transport of Messenger RNA into Different Classes of Membrane-Associated Polyribosomes in Ehrlich-Ascites-Tumor Cells
| Content Provider | Scilit |
|---|---|
| Author | Venrooij, Walther J. Gielkens, Arno L. J. Janssen, Albert P. M. Bloemendal, Hans |
| Copyright Year | 1975 |
| Description | Journal: Journal of Biological Inorganic Chemistry The membrane-bound polyribosomes in Ehrlich ascites tumor cells can be separated into a loosely bound and a tightly bound fraction by means of a high salt treatment. Both membrane fractions as well as the free polyribosomes in the supernatant synthesize about the same set of proteins, suggesting a close relationship between these polyribosome fractions in the Ehrlich cell. Relatively high concentrations of cycloheximide do not prevent newly synthesized poly(A)-containing mRNA from entering the tightly bound polyribosome fraction. Nor had treatment of the cells with puromycin in the presence of cycloheximide, which released about 70% of the nascent chains, any significant effect on the entrance of newly synthesized mRNA into tightly bound polyribosomes. These results suggest that in ehrlich ascites tumor cells nascent polypeptide chains are not involved in the binding of polyribosomes to membranes. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1975.tb02225.x/pdf |
| Ending Page | 238 |
| Page Count | 10 |
| Starting Page | 229 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1975.tb02225.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 56 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1975-08-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Cell Biology Bound Polyribosomes Tightly Bound Tumor Cells Ehrlich Ascites Tumor |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |