NDLI: Defective Assembly of the Small Ribosomal Subunit in a Temperature-Sensitive Mutant of Escherichia coli. Experiments in vitro

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Defective Assembly of the Small Ribosomal Subunit in a Temperature-Sensitive Mutant of Escherichia coli. Experiments in vitro

Content Provider	Scilit
Author	Hayes, Françoise Schmitt, Sophie
Copyright Year	1980
Description	Journal: Journal of Biological Inorganic Chemistry Comparison of the properties in vitro of total 30-S ribosomal subunit proteins and purified protein S4 of Escherichia coli D10 (wild type) and E. coli 219ts2(temperature-sensitive) has given the following results. 1. Reconstitution of functional 30-S subunits in vitro occurs when total 30-S subunit proteins of either strain are used even at temperatures which are not permissive for 30-S subunit assembly in vivo in E. coli 219ts2. The yield of reconstitution is, however, twofold lower with 30-S subunit proteins of E. coli 219ts2 than with wild-type proteins. 2. The yield of complex formation between 16-S rRNA and protein S4 of E. coli 219ts2 is temperature-sensitive and lower at all temperatures tested (33-42 degrees C) than that observed when wild-type S4 is used. 3. The conformational stability of complexes between 16-S rRNA and S4 from 219ts2 is more temperature-sensitive than that of analogous complexes containing wild-type S4. These observations provide an explanation for the temperature sensitivity of 30-S subunit assembly in E. coli 219ts2.
Ending Page	103
Starting Page	95
ISSN	2573508X
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1980.tb04629.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	1
Volume Number	107
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1980-06-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Escherichia Coli Ribosomal Subunit Small Ribosomal Sensitive Mutant Defective Assembly
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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