NDLI: Subunit Composition of Escherichia coli RNA Polymerase during Transcription in vitro

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Subunit Composition of Escherichia coli RNA Polymerase during Transcription in vitro

Content Provider	Scilit
Author	Bordier, Clement Rossetti, Giuseppe Plinio
Copyright Year	1976
Description	Journal: Journal of Biological Inorganic Chemistry The subunit composition of Escherichia coli RNA polymerase during the transcription in vitro of bacteriophage T7DNA was analysed at several steps of RNA synthesis. RNA-polymerase . DNA complexes were sedimented through a glycerol gradient and the RNA polymerase subunits present in each fraction of the gradient were separated by dodecylsulfate-polyacrylamide gel electrophoresis and quantified colorimetrically on the gels. RNA polymerase selectively bound to T7 DNA in the absence of nucleoside triphosphates has the same subunit composition as free RNA polymerase holoenzyme (beta'betaalpha2) omicron. Addition of the nucleoside triphosphate combinations ATP, GTP, UTP or ATP, CTP, UPT, or GTP, CTP UTP to the binding reaction does not alter the subunit composition of RNA polymerase holenzyme bound to DNA. In contrast, in the presence of ATP, GTP and CTP up to 3 pmol of omicron-subunit are released from a complex containing RNA polymerase and 1 pmol of T7 DNA. In the presence of the four nucleoside triphosphates about 90% of the RNA polymerase associated with DNA and nascent RNA has the subunit composition of RNA polymerase core enzyme (bet'betaalpha2). The omicron-subunit is released from the complex and is recovered near the top of the gradient. The transition from the binding complex to the elongation complex and the incorporation of gamma32P-labeled ATP and GTP at the 5' end of RNA molecules were followed in parallel. In the purified elongation complex about 1 pmol of ATP or GTP is incorporated into RNA per pmol RNA polymerase core enzyme engaged in RNA synthesis.
Ending Page	153
Starting Page	147
ISSN	2573508X
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1976.tb10399.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	1
Volume Number	65
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1976-05-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Subunit Composition
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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