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Heterogeneity of Native Ribosomal 60-S Subunits in Ehrlich Ascites Tumor Cells Cultured in vitro
| Content Provider | Scilit |
|---|---|
| Author | Venrooij, Walther J. Janssen, Albert P. M. |
| Copyright Year | 1976 |
| Description | Journal: Journal of Biological Inorganic Chemistry Native large ribosomal subunits in cultured Ehrlich ascites tumor cells analyzed by high-resolution CsCl isopycnic centrifugation consist of at least two classes of particles with densities of 1.57 g/cm3 (LI) and 1.59 g/cm3 (LII), respectively. A wash with 0.5 M KCl converts LI into particles with the density of LII particles. Incubation of derived large subunits (density 1.59 g/cm3) with 0.5 M KCl wash of reticulocyte ribosomes leads to the formation of particles with the density of LI particles. A protein with a molecular weight of 57000 present in the high-KCl wash of 60-S native subunits was virtually absent in the KCl wash of 40-S subunits or polyribosomes suggesting that specific protein factors may be present on some native 60-S subunits. Possible functions of these protein factors are discussed. |
| Ending Page | 60 |
| Starting Page | 55 |
| ISSN | 2573508X |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1976.tb10857.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 69 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1976-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
