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Characterization of $[^{3}$H]Ouabain Binding Sites in Human Brain, Platelet, and Erythrocyte
| Content Provider | Scilit |
|---|---|
| Author | Hauger, R. Luu, H. M. D. Goodwin, F. K. Paul, S. M. |
| Copyright Year | 1985 |
| Description | Journal: Journal of Neurochemistry [3H]Ouabain binding was investigated in membranes prepared from human brain, erythrocyte, and platelet. Scatchard analysis of [3H]ouabain binding to human hypothalamic membranes revealed a single class of noninteracting binding sites with an apparent affinity constant (KD) of 21 nM. Though the number of [3H]ouabain binding sites was lower in human platelets than in erythrocytes, both tissues exhibited a single class of high-affinity binding sites with an apparent KD similar to that found in human brain. Specific [3H]ouabain binding in basal ganglia tissue from patients with Huntington's disease was more than 50% lower than in tissue from age- and sex-matched controls. These results, along with previous findings in rat brain, suggest that high-affinity [3H]ouabain binding labels the neuronal form of Na, K-ATPase in human brain, and may prove useful in quantitating this enzyme in postmortem brain samples. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1985.tb07157.x/pdf |
| Ending Page | 1708 |
| Page Count | 5 |
| Starting Page | 1704 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1985.tb07157.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 6 |
| Volume Number | 44 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1985-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Central Nervous System Huntington's Disease |
| Content Type | Text |
| Resource Type | Article |
