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A Comparison of the Proteins of the Amoebal and Plasanodial Phases of the Slime Mould, Physarum polycephalum
| Content Provider | Scilit |
|---|---|
| Author | Turnock, Geoffrey Morris, Stephen R. Dee, Jennifer |
| Copyright Year | 2005 |
| Description | Journal: Journal of Biological Inorganic Chemistry 1. The proteins of amoebae and plasmodia of strain CL of Physarum polycephalum have been compared by two-dimensional gel electrophoresis. Both forms of the organism were labelled by growth on formalin-killed bacteria labelled with [35S]sulphate, [3H]lysine or [14C]lysine. Plasmodia were also labelled from radioactive lysine in the medium. 2. Of 306 relatively abundant proteins examined, 26% were phase-specific, that is they were found only in amoebae or in plasmodia. About a quarter of these apparent differences in gene expression may be due to minor changes in charge and/or size. 3. Amongst the 74% of the proteins present in both amoebae and plasmodia, there are substantial differences in differential rates of synthesis and these have been measured for a representative set of proteins by a double-label procedure. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1981.tb06235.x/pdf |
| Ending Page | 538 |
| Page Count | 6 |
| Starting Page | 533 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1981.tb06235.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 3 |
| Volume Number | 115 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2005-03-03 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemical Research Amoebae and Plasmodia Physarum Polycephalum |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
