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The Organisation of Genes for Transfer RNA and Ribosomal RNA in Amoebae and Plasmodia of Physarum polycephalum
| Content Provider | Scilit |
|---|---|
| Author | Hall, Len Braun, Richard |
| Copyright Year | 1977 |
| Description | Journal: Journal of Biological Inorganic Chemistry 1. Using hybridisation techniques nuclei from both amoebae and plasmodia of Physarum polycephalum were found to contain 275 genes each coding for 5.8-S, 19-S and 26-S rRNA, 685 genes for 5-S rRNA and 1050 genes for tRNA. 2. Hybridisation of these RNA species to both amoebal and plasmodial DNA fractionated on CsCl gradients reveal that the 5.8-S, 19-S and 26-S rRNA genes are located at a satellite position (formula: see text) with respect to the main band of DNA, whereas 4-S RNA genes are located exclusively in the main band of DNA (formula: see text). 3. This result was confirmed by demonstrating that only the 5.8-S, 19-S and 26-S rRNA species hybridise to purified plasmodial ribosomal DNA. 4. The 19-S and 26-S rRNA genes of amoebae are located on extrachromosomal DNA molecules of a discrete size (Mr = 38 X 10(6)) with identical properties to plasmodial ribosomal DNA. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1977.tb11582.x/pdf |
| Ending Page | 174 |
| Page Count | 10 |
| Starting Page | 165 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1977.tb11582.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 76 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1977-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemical Research |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
