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Synthesis of homologous peptides using fragment condensation: analogs of an HIV proteinase substrate
| Content Provider | Scilit |
|---|---|
| Author | Bláha, Ivo Nemec, Josef Tözsér, József Oroszlan, Stephen |
| Copyright Year | 2009 |
| Description | Journal: International journal of peptide and protein research Two protected peptides Boc-Val-Ser(Bzl)-Gln-Asn-Tyr(BrZ)OH and Boc-Val-Ser(Bzl)-Gln-Asn-Tyr(BrZ)-ProOH were synthesized on a resin substituted by 9-(hydroxymethyl)-2-fluoreneacetic acid. After cleavage with piperidine/DMF, desalting, and activation, these peptides were used for the synthesis of 11 analogs of an HIV proteinase nonapeptide substrate Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-NH2 using fragment condensation in solid phase. The fragment condensation was made in an ultrasonic bath. Using only 2 equivalents of the activated peptide in a DMF solution, this reaction was complete in 2 h. All nonapeptides were assayed as substrates for HIV-1 and HIV-2 proteinases. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1399-3011.1991.tb01526.x/pdf |
| Ending Page | 458 |
| Page Count | 6 |
| Starting Page | 453 |
| ISSN | 03678377 |
| DOI | 10.1111/j.1399-3011.1991.tb01526.x |
| Journal | International journal of peptide and protein research |
| Issue Number | 5 |
| Volume Number | 38 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2009-01-12 |
| Access Restriction | Open |
| Subject Keyword | Journal: International journal of peptide and protein research Fragment Condensation Hiv Proteinase Solid Phase Peptide Synthesis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry |
