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Differentiation-dependent and cell-specific regulation of the hIGFBP-1 gene in human endometrium.
| Content Provider | Scilit |
|---|---|
| Author | Tseng, Linda Gao, Jiaguo Mazella, James Zhu, Hui Hui Lane, Bernard |
| Copyright Year | 1997 |
| Description | Journal: Annals of the New York Academy of Sciences We analyzed IGFBP-1 gene promoter activity by transient transfection during the progressive decidualization of human endometrial stromal cells. A time study over a 13-day culture period showed that the promoter activity increased exponentially to > 10(4) fold in cells treated with MPA and RLX correlating with the secretion rate and steady-state mRNA levels of the endogenous gene. Deletion analysis showed that two regions in the IGFBP-1 gene promoter are responsible for the activation of the IGFBP-1 gene. The basal promoter region between -1 and -300 bp contains multiple sections of functional elements homologous either to CRE, PRE, or CCAAT. The major difference of IGFBP-1 gene activation in endometrium and the hepatic system lies in the distal promoter region, between -2.6 and -3.4 kb, which mediates 95% of the total promoter activity derived from -3.3 kb to +68 bp. Functional and binding analysis in the distal promoter region showed that multiple Sp1 elements interacting with a novel Sp3 transcription factor activates the hIGFBP-1 gene promoter. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.1997.tb48521.x/pdf |
| Ending Page | 37 |
| Page Count | 11 |
| Starting Page | 27 |
| e-ISSN | 17496632 |
| DOI | 10.1111/j.1749-6632.1997.tb48521.x |
| Journal | Annals of the New York Academy of Sciences |
| Issue Number | 1 |
| Volume Number | 828 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1997-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Annals of the New York Academy of Sciences Biochemistry and Molecular Biology Reproductive Biology Transcription Factor Promoter Activity |
| Content Type | Text |
| Resource Type | Article |
