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Rapid and Sensitive PCR Detection ofVibrio trachuriPathogenic to Japanese Horse Mackerel (Trachurus japonicus)
| Content Provider | Scilit |
|---|---|
| Author | Iwamoto, Yoshihisa Suzuki, Yusaku Kurita, Akinobu Watanabe, Yoko Shimizu, Tadayori Ohgami, Hirohisa Yanagihara, Yasutake |
| Copyright Year | 1995 |
| Description | Journal: Microbiology and Immunology A polymerase chain reaction (PCR) method was performed for rapid and sensitive detection of pathogenic Vibrio trachuri isolated from cultured Japanese horse mackerel. A set of primers was selected from the base sequence of the Pst I fragment of T9210 chromosomal DNA and used for PCR detection of T9210. This PCR specifically amplified the DNAs from V. trachuri T9210, T9213, and T9216 but not of those other bacterial strains. PCR using a Pst I-1 primer set made it possible to detect 100 fg of T9210 DNA. The PCR method reported here may be useful for detection and identification of V. trachuri pathogenic to Japanese horse mackerel. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.1995.tb03290.x/pdf |
| Ending Page | 1006 |
| Page Count | 4 |
| Starting Page | 1003 |
| ISSN | 03855600 |
| e-ISSN | 13480421 |
| DOI | 10.1111/j.1348-0421.1995.tb03290.x |
| Journal | Microbiology and Immunology |
| Issue Number | 12 |
| Volume Number | 39 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1995-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Microbiology and Immunology Metallurgy and Metallurgical Engineering Polymerase Chain Reaction Vibrio Trachuri Japanese Horse Mackerel Pst I Fragment |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Immunology Microbiology |
