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Evolutionary divergence of photoprotection in the green algal lineage: a plant‐like violaxanthin de‐epoxidase enzyme activates the xanthophyll cycle in the green alga Chlorella vulgaris modulating photoprotection
| Content Provider | Scilit |
|---|---|
| Author | D'Andrea, Cosimo Girolomoni, Laura Bellamoli, Francesco Valbuena, Gabriel Cruz Perozeni, Federico Cerullo, Giulio Cazzaniga, Stefano Ballottari, Matteo |
| Copyright Year | 2020 |
| Description | Journal: New Phytologist The xanthophyll cycle is the metabolic process by which the carotenoid violaxanthin is de‐epoxidated to zeaxanthin, a xanthophyll with a crucial photoprotective role in higher plants and mosses. In green algae the role of zeaxanthin is still unclear and a peculiar violaxanthin de‐epoxidating enzyme was found in the model organism Chlamydomonas reinhardtii. Here, we investigated the molecular details and functions of the xanthophyll cycle in the case of Chlorella vulgaris, one of the most considered green algae for industrial cultivation, where resistance to high light stress is a pre‐requisite for sustainable biomass production. Identification of the violaxanthin de‐epoxidase enzyme in Chlorella vulgaris was performed by genome mining and in vitro analysis of the catalytic activity of the identified gene product. The photoprotective role of zeaxanthin was then investigated in vivo and in isolated pigment binding complexes. The obtained results demonstrate the functioning, even though with a different pH sensitivity, of a plant‐like violaxanthin de‐epoxidase enzyme in Chlorella vulgaris . Differently from Chlamydomonas reinhardtii , zeaxanthin accumulation in Chlorella vulgaris was found to be crucial for photoprotective quenching of excitation energy harvested by both Photosystem I and II. These findings demonstrate an evolutionary divergence of photoprotective mechanisms among Chlorophyta . |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539987/pdf |
| Ending Page | 150 |
| Page Count | 15 |
| Starting Page | 136 |
| e-ISSN | 14698137 |
| DOI | 10.1111/nph.16674 |
| Journal | New Phytologist |
| Issue Number | 1 |
| Volume Number | 228 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2020-05-22 |
| Access Restriction | Open |
| Subject Keyword | Journal: New Phytologist Chlorella Vulgaris Non‐photochemical Quenching Xanthophyll Cycle |
| Content Type | Text |
| Resource Type | Article |
