NDLI: Cyclic AMP Accumulation Induces a Rapid Desensitization of the Cyclic AMP-Dependent Protein Kinase in Mouse Striatal Neurons

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Cyclic AMP Accumulation Induces a Rapid Desensitization of the Cyclic AMP-Dependent Protein Kinase in Mouse Striatal Neurons

Content Provider	Scilit
Author	Chneiweiss, Hervé Cordier, Jocelyne Glowinski, Jacques
Copyright Year	1991
Description	Journal: Journal of Neurochemistry Striatal neurons from the mouse brain embryo grown in primary culture express high levels of cyclic AMP (cAMP)-dependent protein kinase (PKA) activity. To study the modulation of PKA in intact neurons, a rapid method based on Zn(2+)-protein precipitation was developed. This strategy allowed analysis of the stimulation of PKA under conditions of intracellular cAMP concentration increases. Whereas increases up to 1 microM lead to an activation, large and sustained accumulations of cAMP result in a loss of the enzyme activity. With 8-bromo-cAMP (8-Br-cAMP) at 100 microM, the PKA refractoriness occurs within 2 min. It is rapidly reversible because incubation of treated neurons in fresh medium leads to a complete recovery of PKA activity within 30 min. The decrease in assayable PKA does not involve an activation of phosphatases because the histone dephosphorylation rate is not affected by 8-Br-cAMP treatment. Finally, not only 8-Br-cAMP- but also forskolin- and vasoactive intestinal peptide-induced increases in intracellular cAMP concentration can lead to the PKA desensitization. Altogether, these data unravel a new mechanism of PKA regulation.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1991.tb06371.x/pdf
Ending Page	1715
Page Count	8
Starting Page	1708
e-ISSN	14714159
DOI	10.1111/j.1471-4159.1991.tb06371.x
Journal	Journal of Neurochemistry
Issue Number	5
Volume Number	57
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1991-11-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Neurochemistry Biochemistry and Molecular Biology Cyclic Amp‐dependent Protein Kinase
Content Type	Text
Resource Type	Article

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