NDLI: Phosphorylation of Endogenous Proteins by Adenosine 3':5'-Monophosphate-Dependent Protein Kinase in Mouse Neuroblastoma Cells

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Phosphorylation of Endogenous Proteins by Adenosine 3':5'-Monophosphate-Dependent Protein Kinase in Mouse Neuroblastoma Cells

Content Provider	Scilit
Author	Prashad, Nagindra Evetts, Curtis Wischmeyer, Betsy
Copyright Year	1980
Description	Journal: Journal of Neurochemistry Increased intracellular adenosine 3':5'-monophosphate (cAMP) levels and activation of cAMP-dependent protein kinases (ATP:protein phosphotransferase, EC 2.7.1.37) in vivo were correlated in mouse neuroblastoma cells grown in the presence of 1 mM-6N,O2'-dibutyryl 3':5'-monophosphate (Bt2cAMP). The time course for activation showed that cAMP-dependent protein kinases were activated by 30 min. A heat-stable inhibitor protein inhibited a majority of activated cAMP-dependent protein kinase. Activation of cAMP-dependent protein kinase caused additional phosphorylation of proteins when compared with untreated control cells, as demonstrated by endogenous phosphorylation of proteins in vitro using [gamma-32P]ATP and analysis by two-dimensional polyacrylamide gel electrophoresis. The phosphorylation data show selective phosphorylation of specific proteins by cAMP-independent and cAMP-dependent protein kinase. Among the proteins in the postmitochondrial supernatant fraction phosphorylated by cAMP-dependent protein kinases, two proteins with a molecular weight of 43,000 were heavily phosphorylated. It is suggested that phosphorylation of cellular proteins by cAMP-dependent protein kinases might be involved in the cAMP-modulated biochemical changes in neuroblastoma cells.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1980.tb12487.x/pdf
Ending Page	46
Page Count	9
Starting Page	38
e-ISSN	14714159
DOI	10.1111/j.1471-4159.1980.tb12487.x
Journal	Journal of Neurochemistry
Issue Number	1
Volume Number	35
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1980-07-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Neurochemistry Biochemical Research Biochemistry and Molecular Biology Cyclic Amp‐dependent Protein Kinase Protein Inhibitor
Content Type	Text
Resource Type	Article

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