Loading...
Please wait, while we are loading the content...
Similar Documents
Pharmacological characterization of inhibitory effects of postsynaptic opioid and cannabinoid receptors on calcium currents in neonatal rat nucleus tractus solitarius
| Content Provider | Scilit |
|---|---|
| Author | Endoh, Takayuki |
| Copyright Year | 2006 |
| Description | Journal: British Journal of Pharmacology 1. The profile of opioid and cannabinoid receptors in neurons of the nucleus tractus solitarius (NTS) has been studied using the whole-cell configuration of the patch clamp technique. 2. Experiments with selective agonists and antagonists of opioid, ORL and cannabinoid receptors indicated that mu-opioid, kappa-opioid, ORL-1 and CB1, but not delta-opioid, receptors inhibit VDCCs in NTS. 3. Application of [D-Ala2, N-Me-Phe4, Gly5-ol]-enkephalin (DAMGO; mu-opioid receptor agonist), Orphanin FQ (ORL-1 receptor agonist) and WIN55,122 (CB1 receptor agonist) caused inhibition of I(Ba) in a concentration-dependent manner, with IC50's of 390 nM, 220 nM and 2.2 microM, respectively. 4. Intracellular dialysis of the G(i)-protein antibody attenuated DAMGO-, Orphanin FQ- and WIN55,122-induced inhibition of I(Ba). 5. Both pretreatment with adenylate cyclase inhibitor and intracellular dialysis of the protein kinase A (PKA) inhibitor attenuated WIN55,122-induced inhibition of I(Ba) but not DAMGO- and Orphanin FQ-induced inhibition. 6. Mainly N- and P/Q-type VDCCs were inhibited by both DAMGO and Orphanin FQ, while L-type VDCCs were inhibited by WIN55,122. 7. These results suggest that mu- and kappa-opioid receptors and ORL-1 receptor inhibit N- and P/Q-type VDCCs via G alpha(i)-protein betagamma subunits, whereas CB1 receptors inhibit L-type VDCCs via G alpha(i)-proteins involving PKA in NTS. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1616990/pdf |
| Ending Page | 401 |
| Page Count | 11 |
| Starting Page | 391 |
| e-ISSN | 14765381 |
| DOI | 10.1038/sj.bjp.0706623 |
| Journal | British Journal of Pharmacology |
| Issue Number | 4 |
| Volume Number | 147 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2006-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: British Journal of Pharmacology Cannabinoid Receptor Opioid Peptides |
| Content Type | Text |
| Resource Type | Article |
