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Binding and functional activity of nicotinic cholinergic receptors in selected rat brain regions are increased following long-term but not short-term nicotine treatment
| Content Provider | Scilit |
|---|---|
| Author | Nguyen, Henry N. Rasmussen, Bruce A. Perry, David C. |
| Copyright Year | 2004 |
| Description | Journal: Journal of Neurochemistry Chronic nicotine exposure up-regulates neuronal nicotinic receptors, but the functional consequences for these receptors is less well understood. Following 2 weeks of nicotine or saline treatment by osmotic minipump, the functional activity of nicotinic receptors was measured by concentration-response curves for epibatidine-stimulated (86)Rb efflux. Nicotine-treated animals had a significantly higher maximal efflux in cerebral cortex and superior colliculus, but not in thalamus or interpeduncular nucleus plus medial habenula. This increase was confirmed in a separate experiment with stimulation by single concentrations of epibatidine (cortex, superior colliculus) or nicotine (cortex only). Chronic nicotine did not alter (86)Rb efflux stimulated by cytisine, an alpha3beta4-selective agonist, or by potassium chloride, in any region. Short-term (16 h) nicotine exposure caused no changes in either (86)Rb efflux or receptor binding measured with [(3)H]epibatidine. Binding was significantly increased after 2 weeks nicotine exposure in cortex, superior colliculus and thalamus, but not in interpeduncular nucleus plus medial habenula. The increases in epibatidine-stimulated (86)Rb efflux in the four regions tested was linearly correlated with the increases in [(3)H]epibatidine binding in these regions (R(2) = 0.91), suggesting that rat brain receptors up-regulated by chronic nicotine are active. These results have important consequences for understanding nicotinic receptor neurobiology in smokers and users of nicotine replacement therapy. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2004.02482.x/pdf |
| Ending Page | 49 |
| Page Count | 10 |
| Starting Page | 40 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.2004.02482.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 1 |
| Volume Number | 90 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2004-05-25 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Nicotine Dependence Nicotine Replacement Therapy |
| Content Type | Text |
| Resource Type | Article |
