NDLI: Detection of bovine Clostridium perfringens by polymerase chain reaction

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Detection of bovine Clostridium perfringens by polymerase chain reaction

Content Provider	Scilit
Author	Piatti, R. M. Ikuno, A. A. Baldassi, L.
Copyright Year	2004
Description	A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types
Related Links	http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1678-91992004000200005&lng=en&nrm=iso&tlng=en
Ending Page	160
Page Count	7
Starting Page	154
e-ISSN	16789199
DOI	10.1590/s1678-91992004000200005
Journal	Journal of Venomous Animals and Toxins including Tropical Diseases
Issue Number	2
Volume Number	10
Language	English
Publisher	FapUNIFESP (SciELO)
Publisher Date	2004-01-01
Access Restriction	Open
Subject Keyword	Journal of Venomous Animals and Toxins including Tropical Diseases Agriculture and Animal Production Polymerase Chain Reaction Clostridium Perfringens
Content Type	Text
Resource Type	Article

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