Loading...
Please wait, while we are loading the content...
Similar Documents
Clostridium perfringens isolate typing by multiplex PCR
| Content Provider | Scilit |
|---|---|
| Author | Shamsaddini, Mb Ahsani Mohammadabadi |
| Copyright Year | 2010 |
| Description | Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were identified through biochemical tests. DNA extracted from isolated bacteria for genotyping was tested by multiplex PCR with specific primers. Based on length of synthesized fragments by PCR, toxin types and bacterial strains were detected. C. perfringens isolated types were divided as follows: 17.39% type A, 21.74% type B, 34.78% type C and 26.09% type D. It should be emphasized that, up to the present moment, C. perfringens type A has not been reported in Iran. |
| Related Links | http://www.scielo.br/pdf/jvatitd/v16n4/06.pdf http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1678-91992010000400006&lng=en&nrm=iso&tlng=en |
| Ending Page | 578 |
| Page Count | 6 |
| Starting Page | 573 |
| e-ISSN | 16789199 |
| DOI | 10.1590/s1678-91992010000400006 |
| Journal | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Issue Number | 4 |
| Volume Number | 16 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2010-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal of Venomous Animals and Toxins including Tropical Diseases Agriculture and Animal Production Clostridium Perfringens Multiplex Pcr Biochemical Tests Genetic Typing |
| Content Type | Text |
| Resource Type | Article |
