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Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
| Content Provider | Scilit |
|---|---|
| Author | Khan, Abdul Basit Khattak, Mehmood Iqbal Tarar, Omer Mukhtar Habib, Farzana Jamil, Khalid Yasmin, Arfa Parvez, Shoukat |
| Copyright Year | 2013 |
| Description | In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR |
| Related Links | http://www.scielo.br/pdf/babt/v56n4/15.pdf http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1516-89132013000400015&lng=en&nrm=iso&tlng=en |
| Ending Page | 652 |
| Page Count | 8 |
| Starting Page | 645 |
| ISSN | 15168913 |
| e-ISSN | 16784324 |
| DOI | 10.1590/s1516-89132013000400015 |
| Journal | Brazilian Archives of Biology and Technology |
| Issue Number | 4 |
| Volume Number | 56 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2013-08-01 |
| Access Restriction | Open |
| Subject Keyword | Brazilian Archives of Biology and Technology Biotechnology and Microbiology Polyhydroxyalkanoates Biodegradable Polymers Pha Synthase Gene |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
