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Desenvolvimento e validação de método por cromatografia líquida de alta eficiência para determinação simultânea das impurezas timina e timidina na matéria-prima estavudina
| Content Provider | Scilit |
|---|---|
| Author | Silva, Gisele Rodrigues da Condessa, Felipe Antonacci Pianetti, Gérson Antônio de Aguiar Nunan, Elzíria de Campos, Ligia Maria Moreira |
| Copyright Year | 2008 |
| Description | A HPLC method was developed to quantify thymine and thymidine impurities in stavudine bulk drug. The separation was carried out in isocratic mode using methanol/water (20:80) as mobile phase, a C18 column and UV detection at 266 nm. The method provided selectivity based on peak purities and resolution among peaks. It was linear over the range of 0.5-5.0 µg/mL. The quantitation limits were 0.021 µg/mL for thymine and 0.134 µg/mL for thymidine. The average accuracies of three concentrations ranged from 97.06 to 102.61% and precision was close to 1%. The method showed robustness, remaining unaffected by deliberate variations in relevant parameters |
| Related Links | http://www.scielo.br/pdf/qn/v31n7/v31n7a16.pdf |
| Ending Page | 1690 |
| Page Count | 5 |
| Starting Page | 1686 |
| ISSN | 01004042 |
| e-ISSN | 01004042 |
| DOI | 10.1590/s0100-40422008000700016 |
| Journal | Química Nova |
| Issue Number | 7 |
| Volume Number | 31 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2008-01-01 |
| Access Restriction | Open |
| Subject Keyword | Química Nova |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry |
