NDLI: USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila

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USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila

Content Provider	Scilit
Author	Ristic, Gorica Tsou, Wei-Ling Guzi, Ermal Kanack, Adam J. Scaglione, Kenneth Matthew Todi, Sokol V.
Copyright Year	2016
Description	Journal: Journal of Biological Chemistry Ubiquitination is a post-translational modification that regulates most cellular pathways and processes, including degradation of proteins by the proteasome. Substrate ubiquitination is controlled at various stages, including through its reversal by deubiquitinases (DUBs). A critical outcome of this process is the recycling of monoubiquitin. One DUB whose function has been proposed to include monoubiquitin recycling is USP5. Here, we investigated whether Drosophila USP5 is important for maintaining monoubiquitin in vivo. We found that the fruit fly orthologue of USP5 has catalytic preferences similar to its human counterpart and that this DUB is necessary during fly development. Our biochemical and genetic experiments indicate that reduction of USP5 does not lead to monoubiquitin depletion in developing flies. Also, introduction of exogenous ubiquitin does not suppress developmental lethality caused by loss of endogenous USP5. Our work indicates that a primary physiological role of USP5 is not to recycle monoubiquitin for reutilization, but that it may involve disassembly of conjugated ubiquitin to maintain proteasome function.
Related Links	http://www.jbc.org/content/291/17/9161.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861482/pdf http://www.jbc.org/article/S0021925820411159/pdf
Ending Page	9172
Page Count	12
Starting Page	9161
ISSN	00219258
e-ISSN	1083351X
DOI	10.1074/jbc.m115.703504
Journal	Journal of Biological Chemistry
Issue Number	17
Volume Number	291
Language	English
Publisher	Elsevier BV
Publisher Date	2016-04-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Chemistry 70 Kilodalton Heat Shock Protein (hsp70) Deubiquitylation (deubiquitination) Post-translational Modification (ptm)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila

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USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila