NDLI: Structural and Biological Interaction of hsc-70 Protein with Phosphatidylserine in Endosomal Microautophagy

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Structural and Biological Interaction of hsc-70 Protein with Phosphatidylserine in Endosomal Microautophagy

Content Provider	Scilit
Author	Morozova, Kateryna Clement, Cristina C. Kaushik, Susmita Stiller, Barbara Arias, Esperanza Ahmad, Atta Rauch, Jennifer N. Chatterjee, Victor Melis, Chiara Scharf, Brian Gestwicki, Jason E. Cuervo, Ana-Maria Zuiderweg, Erik R. P. Santambrogio, Laura
Copyright Year	2016
Description	Journal: Journal of Biological Chemistry Hsc-70 (HSPA8) is a cytosolic molecular chaperone, which plays a central role in cellular proteostasis, including quality control during protein refolding and regulation of protein degradation. hsc-70 is pivotal to the process of macroautophagy, chaperone-mediated autophagy, and endosomal microautophagy. The latter requires hsc-70 interaction with negatively charged phosphatidylserine (PS) at the endosomal limiting membrane. Herein, by combining plasmon resonance, NMR spectroscopy, and amino acid mutagenesis, we mapped the C terminus of the hsc-70 LID domain as the structural interface interacting with endosomal PS, and we estimated an hsc-70/PS equilibrium dissociation constant of 4.7 ± 0.1 μm. This interaction is specific and involves a total of 4–5 lysine residues. Plasmon resonance and NMR results were further experimentally validated by hsc-70 endosomal binding experiments and endosomal microautophagy assays. The discovery of this previously unknown contact surface for hsc-70 in this work elucidates the mechanism of hsc-70 PS/membrane interaction for cytosolic cargo internalization into endosomes.
Related Links	http://www.jbc.org/content/291/35/18096.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000059/pdf http://www.jbc.org/article/S0021925820321748/pdf
Ending Page	18106
Page Count	11
Starting Page	18096
ISSN	00219258
e-ISSN	1083351X
DOI	10.1074/jbc.m116.736744
Journal	Journal of Biological Chemistry
Issue Number	35
Volume Number	291
Language	English
Publisher	Elsevier BV
Publisher Date	2016-08-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Chemistry 70-kilodalton Heat Shock Protein (hsp70) Phosphatidylserine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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