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Regulation of flagellar motor switching by c-di-GMP phosphodiesterases in Pseudomonas aeruginosa
| Content Provider | Scilit |
|---|---|
| Author | Xin, Lingyi Zeng, Yukai Sheng, Shuo Chea, Rachel Andrea Liu, Qiong Li, Hoi Yeung Yang, Liang Xu, Linghui Chiam, Keng-Hwee Liang, Zhao-Xun |
| Copyright Year | 2019 |
| Description | Journal: Journal of Biological Chemistry The second messenger cyclic diguanylate (c-di-GMP) plays a prominent role in regulating flagellum-dependent motility in the single-flagellated pathogenic bacterium Pseudomonas aeruginosa. The c-di-GMP–mediated signaling pathways and mechanisms that control flagellar output remain to be fully unveiled. Studying surface-tethered and free-swimming P. aeruginosa PAO1 cells, we found that the overexpression of an exogenous diguanylate cyclase (DGC) raises the global cellular c-di-GMP concentration and thereby inhibits flagellar motor switching and decreases motor speed, reducing swimming speed and reversal frequency, respectively. We noted that the inhibiting effect of c-di-GMP on flagellar motor switching, but not motor speed, is exerted through the c-di-GMP–binding adaptor protein MapZ and associated chemotactic pathways. Among the 22 putative c-di-GMP phosphodiesterases, we found that three of them (DipA, NbdA, and RbdA) can significantly inhibit flagellar motor switching and swimming directional reversal in a MapZ-dependent manner. These results disclose a network of c-di-GMP–signaling proteins that regulate chemotactic responses and flagellar motor switching in P. aeruginosa and establish MapZ as a key signaling hub that integrates inputs from different c-di-GMP–signaling pathways to control flagellar output and bacterial motility. We rationalized these experimental findings by invoking a model that postulates the regulation of flagellar motor switching by subcellular c-di-GMP pools. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746464/pdf http://www.jbc.org/content/294/37/13789.full.pdf |
| Ending Page | 13799 |
| Page Count | 11 |
| Starting Page | 13789 |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| DOI | 10.1074/jbc.ra119.009009 |
| Journal | Journal of Biological Chemistry |
| Issue Number | 37 |
| Volume Number | 294 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2019-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Chemistry Biochemistry and Molecular Biology Pseudomonas Aeruginosa (p. Aeruginosa) Cyclic Di-gmp (c-di-gmp) Methyltransferase Phosphodiesterases |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
